منابع مشابه
Cell Interaction Analysis by Imaging Flow Cytometry
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متن کاملFlow Cytometry
Flow cytometry is a technology that has impacted both basic cell biology and clinical medicine in a very significant manner. The essential principle of flow cytometry is that single particles suspended within a stream of liquid are interrogated individually in a very short time as they pass through a light source focused at a very small region. The optical signals generated are mostly spectral ...
متن کاملFlow Cytometry
Because it has been difficult to identify and separate malignant cells in human lymphoid malignancies, we have developed a flow cytometry-based fluorescent in situ hybridization (FISH) technique using immunoglobulin (Ig) heavy chain variable region (V H) gene probes. After obtaining the specific V H gene sequence expressed by the multiple myeloma IM-9 cell line and the malignant cells in five m...
متن کاملMicrofabricated multiple field of view imaging flow cytometry.
The combination of microscopy and flow cytometry enables image based screening of large collections of cells. Despite the proposition more than thirty years ago, adding high resolution wide-field imaging to flow cytometers remains challenging. The velocity of cells in flow cytometry can surpass a meter per second, requiring either sub-microsecond exposure times or other sophisticated photodetec...
متن کاملPhase imaging flow cytometry using a focus-stack collecting microscope.
This letter introduces a fluidics-based focus-stack collecting microscope. A microfluidic device transports cells through the focal plane of a microscope, resulting in an efficient method to collect focus stacks of large collections of single cells. Images from the focus stacks are used to reconstruct the quantitative phase of cells with the transport-of-intensity-equation method. Using the pha...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 2012
ISSN: 0022-1554,1551-5044
DOI: 10.1369/0022155412453052